Volume 5, Issue 2 (2-2019)
Abstract
Aims: Hepatitis B Virus (HBV) has infected more than million hundreds of people worldwide. Hence, a high rate of morbidity and mortality caused by liver-related diseases is due to HBV infection. However, a strong and effective treatment should be based on an accurate and correct diagnostic method. Hence, the present research provided a multidimensional study comparing and analyzing patients’ molecular and serological tests results.
Materials & Methods: In this research, the HBV DNA molecular tests results were studied by examining patients’ gender, age, and HBsAg strip results.
Findings: Among the female patients (29 persons) studied in this research, 55.1% were positive for HBV DNA and HBsAg strip tests, and 17.3% were negative for both tests. Also, among the male patients (44 persons), 65.9% were positive, and 6.8% were negative for both tests.
Conclusion: The present study results shed light on the correlation between the HBV DNA and HBsAg tests. Also, the significance of HBV DNA tests was highlighted for particular diagnostic purposes and for the differentiation and interpretation of the pathophysiological conditions of patients with hepatitis B.
Hessam Mirshahabi, Mohsen Karimi, Farida Behzadian, Farzaneh Sabahi,
Volume 16, Issue 3 (12-2013)
Abstract
Objective: Despite availability of an effective vaccine against hepatitis B virus (HBV), the global prevalence of this virus infection has not diminished significantly. Contrary to numerous other human viruses, HBV does not have the ability to propagate in cell culture. However, infectious virus has been produced by transfection of human hepatoma cells with plasmids that contain full length HBV genome. Generation and optimization of appropriate cell culture systems can help us in demonstrating the quality of genome replication by PCR as well as expression of surface antigen secretion. Interferon stimulating genes (ISGs) are usually produced in response to interferon and can be determined as a measure of response to IFN-therapy. Therefore, in pharmacological studies, in addition to assessing the effects of a medicine on viral determinants of replication, its’ effects on stimulation of various ISGs, as indicators of innate immune responses, can be achieved.
Methods: In this study, we transfected the Huh-7 hepatoma cell line with pCH-9/3091. HBsAg production and viral mRNA transcription were subsequently evaluated. In this system, by using ISGs-specific primers, the ISG mRNAs recognition method was optimized and utilized.
Results: Huh-7 cells supported HBV replication. The peak HBsAg secretion was observed at 72 h post-transfection. By using designed primers for the S and pg/pC regions, transcription and genome replication of the virus was shown. RT-PCR results for ISG production by transfected cells showed no role for HBV in enhancement of ISGs levels in Huh-7 cells.
Conclusion: The results indicated that this system can be used for functional studies of HBV-specific genes as well as assessment of the effects of new drugs or new vaccines. In addition, it may be used to study the mechanisms of drug resistance that have resulted in difficulties in response to HBV antivirals, including IFN-α.