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Showing 4 results for Nano-Curcumin


Volume 13, Issue 1 (3-2022)
Abstract

Abstract
Introduction: Cervical cancer is the fourth most common cancer among women. In recent years, attention has increased to natural products such as curcumin with anti-cancer potential as a therapeutic supplement. However, due to its poor solubility, its clinical use is limited. In this regard, in this study, to improve clinical parameters, the effects of nanocurcumin on the angiogenesis inhibition of cervical cancer were investigated and compared with free curcumin.
Materials and Methods: MTT method was used to evaluate the proliferation of the HeLa cell line with free curcumin and nanocurcumin at different doses and time intervals and the rate of apoptosis was assessed by flow cytometry. Then, the expression of the vascular endothelial growth factor (VEGF-A) gene in HeLa cells was measured by Real-Time PCR and Western blotting, respectively.
Results: According to IC50 for 48 hours in the HeLa cell line, which was 15 μM/ml and 50 μM/ml for nanocurcumin and free curcumin, respectively, the nanocurcumin showed a greater lethal effect. VEGF-A gene expression (p <0.0001) and protein level (p <0.01) were significantly lower following nano-curcumin treatment than free curcumin.
Conclusion: Nanocarrier increased the solubility and further inhibited the proliferation of cervical cancer HeLa cells and was three times more effective than curcumin in inhibiting angiogenesis at the same concentration. Therefore, nanocurcumin can be a good option for drug supplementation along with routine cervical cancer treatment.
Keywords: Cervical cancer, Nanocurcumin, HeLa cell, VEGF-A.

Volume 16, Issue 96 (1-2020)
Abstract

Curcuminoids as a natural antioxidant have many therapeutic activities such as rheumatoid arthritis, multiple sclerosis, Alzheimer's and atherosclerosis. Curcuminoids have very low solubility in the aquatic environment, limiting its potential applications in the food and pharmaceutical industries. In this study, nanocompleted curcuminoids were added to the pasta with different percentages (0, 0.001, 0.005, 0.01, 0.05 and 0.1) and the rheological, physicochemical and sensory properties of the pasta were measured. The results showed that moisture content, ash, protein, and rheological properties of pasta were not significantly different with the addition of nano-micellized curcuminoids. Also, the outflow of solids in the baking water increased and the baking weight decreased. With increasing nanocrystalline curcuminoid percentages, factor L * and a * decreased and factor b * increased. Sensory evaluation by panelists showed no significant difference in aroma, taste and odor but overall acceptance of pasta increased with increasing curcuminoid.
Hadi Shirzad, Maryam Poursangi, Hosein Maghsoudi, Shirin Jalili,
Volume 19, Issue 4 (9-2017)
Abstract

Objective: Fetal hemoglobin is the predominant hemoglobin expressed by gamma globin. However, in adults, fetal hemoglobin normally reduces to very low levels of the total hemoglobin. The increase in levels of fetal hemoglobin can ameliorate the severity of β-hemoglobin disorders such as sickle cell disease and beta-thalassemia. Currently, drugs that have been used for induction of Fetal hemoglobin (HbF) have short-term effects. Dendrosomal nano-curcumin (DNC), which has high solubility and absorption, is able to detect different targets in the cell and affect gene expression. LSD1 is one of the most important gamma globin inhibitors. In this study, we examine the capability of DNC to inhibit the expression of LSD1, GATA1, and FOG1 as well as the increase in gamma globin expression. Methods: We used the K562 cell line for the MTT assay and treatment by DNC. Then the effect of DNC on the increase in expression level of γ-globin, decrease expression level of LSD1 and transcription factors, GATA1 and FOG1was investigated by Real time PCR. Results: Data acquired from gene expression assays indicated that DNC induced gamma globin expression and decreased expressions of LSD1, GATA1, and FOG1 in a time and dose-dependent manner. Conclusion: Inhibition of LSD1, GATA1, and FOG1 expressions via DNC led to increased gamma globin expression. These results showed that DNC could be a promising treatment for beta-thalassemia and sickle cell disorders, and possibly reduce the severity of symptoms of these patients through the induction of fetal hemoglobin.
S.m. Seyed Ahmadian , Sh. Jalili Sh., Hadi Shirzad, M. Sadeghizadeh ,
Volume 22, Issue 4 (10-2019)
Abstract

Aims: Oxidative substances are chemically reactive molecules and a byproduct of oxidative metabolism. Oxidative stress is one of the most lethal mechanisms in the toxicity of heavy metals such as lead. Since curcumin is an active ingredient in turmeric and has many properties, including antioxidant properties, the present study was conducted to evaluate the effect of milk and milk containing nano-curcumin on lead toxicity and to determine the effective concentration of nano-curcumin in controlling lead toxicity.
Materials & Methods: In the present study, the Huh7-1x-ARE-luc cell line, a biosensor of oxidative stress, was treated with 30μM of lead as a strong oxidant. Then the antioxidant effect of low-fat and high-fat milk (20, 40, and 80μL), nano-curcumin in antioxidant concentrations (4 and 8μM) and simultaneous treatment with the combination of these two antioxidants was tested using Luciferase assay.
Results: Based on statistical analyses, the combination of milk and nano-curcumin (combination of 30μM lead, 20μL milk and 4μM nano-curcumin) was able to significantly reduce lead toxicity at low concentrations of milk compared to the milk without nano-curcumin (combination of 30μM lead and 80μL milk), with RLU of 1266 and 34000, respectively.
Discussion & Conclusion: Nano-curcumin reveals a stronger antioxidant effect compared to milk, and ultimately, the combination of nano-curcumin and milk greatly neutralizes lead toxicity.
 


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