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Showing 2 results for Osteogenic Differentiation


Volume 12, Issue 2 (1-2022)
Abstract

 Graphene-based nanomaterials are being investigated for their biocompatibility and bioactivity, as well as their ability to improve osteogenic differentiation. In this research, the base material, reduced graphene oxide (rGO) sheets, were decorated with hydroxyapatite and strontium (rGO / HAp-Sr) to induce osteogenic differentiation in adipose-derived mesenchymal stem cells. Different techniques were used to determine the properties of the nanocomposite such as diffraction analysis techniques (XRD) and transmission electron microscopy (to evaluate the size and morphology of HAp-Sr on rGO plates), FT-IR (to analyze the nanocomposite functional group), Raman spectroscopy (to investigate possible disorders in nanocomposite structure and number of layers), induced dual plasma emission spectroscopy (to assess atomic concentration of Ca and Sr), zeta potential(electrical potential of the nanocomposite) and MTT (nanocomposite cytotoxicity assessment) were used. The ossification potential of the synthesized nanocomposite was investigated and confirmed using the calcium deposition test in dipose-derived mesenchymal stem cells. According to the obtained results, osteogenic differentiation induction is possible using synthesized nanocomposites without the need for chemical inducers.
 
Fatemeh Zolghadr, Majid Sadeghizadeh, Naser Amirizadeh, Mehrdad Behmanesh, Saman Hosseinkhani, Maryam Amani,
Volume 14, Issue 2 (6-2011)
Abstract

Objective: Evaluating the effects of p-benzoquinone and hydroquinone on the RUNX2 expression and osteoblastic differentiation of human marrow derived mesenchymal stem cells (MSCs). Materials and Methods: Bone marrow MSCs obtained by cultivating marrow mononuclear cells, were exposed to 10μM of either p-benzoquinone or hydroquinone. Following chemical treatment, RUNX2 gene expression was assessed by Real-time RT PCR 1, 6, 24 and 48 hours later and osteogenic differentiation was analyzed using alizarin red and alkaline phosphatase staining methods on days 7 and 14 after ostegenic induction. Results: RUNX2 expression was significantly elevated (up to approximately 8 times) due to chemical exposure but the applied chemicals exert no considerable effect on MSCs osteogenic differentiation. Conclusion: According to the literature, despite the necessity of RUNX2 overexpression on the induction of osteogenic differentiation, but it is not sufficient for osteogenesis to occure so increase in RUNX2 expression observed in our study is not the indicator of the induced osteogenic differentiation. Instead, this elevated expression could be the sign of increased activity of the canonical Wnt signaling pathway thereby its involvement in the development of AML due to exposure to benzene and its metabolites. Moreover, this augmented expression of RUNX2 in MSCs can indicate the RUNX2 overexpression in myeloid progenitors as an expected similar effect of exposure to benzene and its metabolites to contribute in myeloid malignancies developed due to benzene exposure.

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