Volume 22, Issue 2 (2019)                   mjms 2019, 22(2): 97-102 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ravanbakhsh Gavgani R, Babaei E, Hosseinpourfeizi M, Fakhrjou A, Montazeri V. Over Expression of Long Non-Coding RNA HULC in Women with Breast Invasive Ductal Carcinoma in East Azerbaijan Province. mjms 2019; 22 (2) :97-102
URL: http://mjms.modares.ac.ir/article-30-25619-en.html
Over Expression of Long Non-Coding RNA HULC in Women with Breast Invasive Ductal Carcinoma in East Azerbaijan Province
R. Ravanbakhsh Gavgani1 , E. Babaei 2, M.A. Hosseinpourfeizi3 , A. Fakhrjou4 , V. Montazeri5
1- Biological Sciences Department, Natural Sciences Faculty, University of Tabriz, Tabriz, Iran
2- Biological Sciences Department, Natural Sciences Faculty, University of Tabriz, Tabriz, Iran , babaei@tabrizu.ac.ir
3- Department of Biological Sciences, School of Natural Sciences, University of Tabriz, Tabriz, Iran,
4- Pathology Department, Medicine Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
5- Thoracic Surgery Department, Medicine Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:   (6699 Views)

Aims: Despite recent advances in diagnosis and treatment, breast cancer still remains the second leading cause of cancer- related death in women. Recent reports have detected a new class of non-coding molecules named long non-coding RNAs that play an important role in various biological processes involved in cancer. This study aimed to evaluate the expression level of long non-coding RNA HULC in breast cancer.
Materials and Methods: In this experimental study, after collecting 40 breast tumors with invasive ductal carcinoma and 40 normal marginal tissues, RNA extraction and cDNA synthesis were done. The expression level of HULC was obtained by using the qRT-PCR method. REST 2009 software was employed to evaluate the association of its expression in tumor and normal tissues. Biomarker potential of HULC was evaluated by drawing ROC curve. Relationship between HULC expression and clinicopathological features was analyzed.
Results: Results from REST indicated significant upregulation of HULC in tumor tissues compared to normal marginal specimens (95% CI; p=0.0001). ROC curve analysis also demonstrated the biomarker potential of HULC in breast cancer (ROCAUC=0.79; p<0.0001). Evaluation of the relationship between HULC expression and clinicopathological features revealed that there is a statistically significant positive correlation of HULC expression with advanced stages (95% CI; P=0.019).
Conclusion: Considering the upregulation of HULC expression in invasive ductal carcinoma, this lncRNA could be considered as a new potential diagnostic biomarker in breast cancer.
 
 

Keywords: Invasive Ductal Carcinoma, Long Non-Coding RNA, HULC, Biomarkers
Full-Text [PDF 645 kb]   (2525 Downloads)    
Article Type: Original Research | Subject: Molecular Biology
Received: 2018/09/30 | Accepted: 2019/03/11
References
1. Smith RA, Andrews KS, Brooks D, Fedewa SA, Manassaram-Baptiste D, Saslow D, et al. Cancer screening in the United States, 2017: a review of current American Cancer Society guidelines and current issues in cancer screening. CA Cancer J Clin. 2017;67(2):100-21. [Link] [DOI:10.3322/caac.21392]
2. Esteller M. Non-coding RNAs in human disease. Nat Rev Genet. 2011;12(12):861-74. [Link] [DOI:10.1038/nrg3074]
3. Gibb EA, Brown CJ, Lam WL. The functional role of long non-coding RNA in human carcinomas. Mol cancer 2011;10(1):38. [Link] [DOI:10.1186/1476-4598-10-38]
4. Peng W, Wang Z, Fan H. LncRNA NEAT1 impacts cell proliferation and apoptosis of colorectal cancer via regulation of Akt signaling. Pathol Oncol Res. 2017; 23(3):651-6. [Link] [DOI:10.1007/s12253-016-0172-4]
5. Liu Y, Sun Z, Zhu J, Xiao B, Dong J, Li X. LncRNA‐TCONS_00034812 in cell proliferation and apoptosis of pulmonary artery smooth muscle cells and its mechanism. J Cell physiol. 2018; 233(6): 4801-14. [Link] [DOI:10.1002/jcp.26279]
6. Brockdorff N. Noncoding RNA and Polycomb recruitment. RNA. 2013; 19(4): 429-42. [Link] [DOI:10.1261/rna.037598.112]
7. Achour C, Aguilo F. Long non-coding RNA and Polycomb: An intricate partnership in cancer biology. 2018; 23:2106-32. [Link] [DOI:10.2741/4693]
8. Gao Y, Chen G, Zeng Y, Zeng J, Lin M, Liu X, Liu J. Invasion and metastasis-related long noncoding RNA expression profiles in hepatocellular carcinoma. Tumour Biol. 2015;36(10):7409-22. [Link] [DOI:10.1007/s13277-015-3408-0]
9. Luo X, Qiu Y, Jiang Y, Chen F, Jiang L, Zhou Y, et al. Long non-coding RNA implicated in the invasion and metastasis of head and neck cancer: Possible function and mechanisms. Mol Cancer. 2018;17(1):14. [Link] [DOI:10.1186/s12943-018-0763-7]
10. Cui M, Xiao Z, Wang Y, Zheng M, Song T, Cai X, Sun B, Ye L, Zhang X. Long noncoding RNA HULC modulates abnormal lipid metabolism in Hepatoma cells through an miR-9-mediated RXRA signaling pathway. Cancer Res. 2015;75(5):846-57. [Link] [DOI:10.1158/0008-5472.CAN-14-1192]
11. Yan H, Tian R, Zhang M, Wu J, Ding M, He J. High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma. Onco Targets Ther. 2017;10:113-20. [Link] [DOI:10.2147/OTT.S124614]
12. Sun X-H, Yang L-B, Geng X-L, Wang R, Zhang Z-C. Increased expression of lncRNA HULC indicates a poor prognosis and promotes cell metastasis in osteosarcoma. Int J Clin Exp Pathol. 2015;8(3):2994-3000. [Link]
13. Qiu M, Xu Y, Wang J, Zhang E, Sun M, Zheng Y, et al. A novel lncRNA, LUADT1, promotes lung adenocarcinoma proliferation via the epigenetic suppression of p27. Cell Death Dis. 2015;6(8):e1858. [Link] [DOI:10.1038/cddis.2015.203]
14. Huang MD, Chen WM, Qi FZ, Xia R, Sun M, Xu TP, et al. Long non-coding RNA ANRIL is upregulated in hepatocellular carcinoma and regulates cell proliferation by epigenetic silencing of KLF2. J Hemat Oncol. 2015;8(1):50-57. [Link] [DOI:10.1186/s13045-015-0153-1]
15. Panzitt K, Tschernatsch MM, Guelly C, Moustafa T, Stradner M, Strohmaier HM, et al. Characterization of HULC, a novel gene with striking up-regulation in hepatocellular carcinoma, as noncoding RNA. Gastroenterology. 2007;132(1):330-42. [Link] [DOI:10.1053/j.gastro.2006.08.026]
16. Peng W, Gao W, Feng J. Long noncoding RNA HULC is a novel biomarker of poor prognosis in patients with pancreatic cancer. Med Oncol. 2014;31(12):346. [Link] [DOI:10.1007/s12032-014-0346-4]
17. Yang Z, Lu Y, Xu Q, Tang B, Park CK, Chen X. HULC and H19 played different roles in overall and disease-free survival from hepatocellular carcinoma after curative hepatectomy: a preliminary analysis from gene expression omnibus. Dis Markers. 2015;2015:191029. [Link] [DOI:10.1155/2015/191029]
18. Zhao Y, Guo Q, Chen J, Hu J, Wang S, Sun Y. Role of long non-coding RNA HULC in cell proliferation, apoptosis and tumor metastasis of gastric cancer: a clinical and in vitro investigation. Oncol Rep. 2014;31(1):358-64. [Link] [DOI:10.3892/or.2013.2850]
19. Wang J, Ma W, Liu Y. Long non-coding RNA HULC promotes bladder cancer cells proliferation but inhibits apoptosis via regulation of ZIC2 and PI3K/AKT signaling pathway. Cancer Biomark. 2017;20(4):425-34. [Link] [DOI:10.3233/CBM-170188]
20. Chen S, Wu DD, Sang XB, Wang LL, Zong ZH, Sun KX, et al. The lncRNA HULC functions as an oncogene by targeting ATG7 and ITGB1 in epithelial ovarian carcinoma. Cell Death Dis. 2017;8(10):e3118. [Link] [DOI:10.1038/cddis.2017.486]
21. Shi F, Xiao F, Ding P, Qin H, Huang R. Long noncoding RNA highly up-regulated in liver cancer predicts unfavorable outcome and regulates metastasis by MMPs in triple-negative breast cancer. Arch Med Res. 2016;47(6):446-53. [Link] [DOI:10.1016/j.arcmed.2016.11.001]
22. Hämmerle M, Gutschner T, Uckelmann H, Ozgur S, Fiskin E, Gross M, et al. Posttranscriptional destabilization of the liver‐specific long noncoding RNA HULC by the IGF2 mRNA‐binding protein 1 (IGF2BP1). Hepatology. 2013;58(5):1703-12. [Link] [DOI:10.1002/hep.26537]
23. Xie H, Ma H, Zhou D. Plasma HULC as a promising novel biomarker for the detection of hepatocellular carcinoma. BioMed Res Int. 2013;2013. [Link] [DOI:10.1155/2013/136106]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.