Volume 23, Issue 3 (2020)                   mjms 2020, 23(3): 129-135 | Back to browse issues page

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Rahimkhani A, Haghighat S, Mahdavi M. Application of α-Tocopherol in the Hepatitis B Vaccine Formulation in order to Improve Humoral and Cellular Immune Responses. mjms 2020; 23 (3) :129-135
URL: http://mjms.modares.ac.ir/article-30-32448-en.html
1- Department of Microbiology, Medical Sciences Branch, Islamic Azad University, Tehran, Iran
2- 2 Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran , mahdavivac@gmail.com
Abstract:   (1530 Views)
Aims: The common hepatitis B vaccination was designed based on alum adjuvant. This adjuvant is failed to stimulate cellular immunity so that, optimization is necessary for better stimulation of cellular immune responses. α--tocopherol is a key component of vitamin E that is considered as immunomodulatory agent to regulate immunity in order to decrease inflammation immune responses. Therefore, in the present study, the effect of α-tocopherol on the improvement of immune responses in hepatitis B vaccination was evaluated.
Materials & Methods: commercial hepatitis B vaccine was formulated with α-tocopherol at the doses of 1, 5, and 10mg. The vaccine was injected into BALB/C mice three times two weeks apart. Ten days after the last injection, blood-drawing was performed from mice groups. The levels of IFN-γ, TNF-α, IL-4, and IL-2 cytokines and IgG total antibody, as well as IgG1 and IgG2a antibody isotypes, were measured by ELISA method.
Findings: Formulated vaccine at the dose of 10mg of α-tocopherol is more potent in the significant increase of IFN-γ, TNF-α, and IL-2 cytokines and low dose result in improvement of humoral immune response.
Conclusion: Immune response of vaccine formulated with α-tocopherol was dose-dependent and at the dose of 10mg demonstrated a significant increase of Th1 response and at the dose of 1mg result in increase of antibody responses.
Full-Text [PDF 415 kb]   (846 Downloads)    
Article Type: Original Research | Subject: Immunology and Allergy
Received: 2019/05/19 | Accepted: 2020/08/4

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