Volume 25, Issue 3 (2022)
mjms 2022, 25(3): 26-31 |
Back to browse issues page
Ethics code: IR.SBMU.MSP.REC.1395.447
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rohani R, Aghaei A, Abdollahifar M, Mirbehbehani H R, Heidari M H. Effect of deep brain stimulation on length of dendrites in hippocampal CA1 neurons in pentylenetetrazol kindled rats. mjms 2022; 25 (3) :26-31
URL: http://mjms.modares.ac.ir/article-30-75003-en.html
URL: http://mjms.modares.ac.ir/article-30-75003-en.html
Razieh Rohani 
1, Abbas Aghaei2 , Mohammad-Amin Abdollahifar2 , Hamid Reza Mirbehbehani3 , Mohammad Hassan Heidari2
1, Abbas Aghaei2 , Mohammad-Amin Abdollahifar2 , Hamid Reza Mirbehbehani3 , Mohammad Hassan Heidari2
1- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , raziehr@yahoo.com
2- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract: (699 Views)
Aim: Applying deep brain stimulation at low-frequency has anticonvulsant effect on kindled seizures. In this study, the effect of low-frequency stimulation (LFS) on kindling-induced changes in the dendritic length of hippocampal CA1 neurons was investigated.
Methods: To administer LFS in the hippocampus, animals first underwent stereotactic surgery and a tripolar electrode were positioned in the CA1 region. Animals received intraperitoneal pentylenetetrazole (PTZ; 34 mg/kg) every other day until they showed three consecutive stage 4 or 5 seizures. LFS was administered to the dorsal hippocampal CA1 area in kindled+LFS group. Hippocampal samples were prepared for stereological assessment one week after termination of LFS application.
Results: PTZ kindling was accompanied with a decrease in dendritic length in CA1 neurons. One week after application of LFS, the length of dendrites was restored to control group values, and there was a significant difference between kindled+LFS and kindled groups. Interestingly, the effect of administering LFS alone in control group, was similar to that of kindled group and a significant decrease was observed in dendritic length.
Conclusion: LFS had a restoring effect on morphological changes in CA1 neurons of kindled animals. This effect may be considered a mechanism for therapeutic action of deep brain stimulation in seizure.
Methods: To administer LFS in the hippocampus, animals first underwent stereotactic surgery and a tripolar electrode were positioned in the CA1 region. Animals received intraperitoneal pentylenetetrazole (PTZ; 34 mg/kg) every other day until they showed three consecutive stage 4 or 5 seizures. LFS was administered to the dorsal hippocampal CA1 area in kindled+LFS group. Hippocampal samples were prepared for stereological assessment one week after termination of LFS application.
Results: PTZ kindling was accompanied with a decrease in dendritic length in CA1 neurons. One week after application of LFS, the length of dendrites was restored to control group values, and there was a significant difference between kindled+LFS and kindled groups. Interestingly, the effect of administering LFS alone in control group, was similar to that of kindled group and a significant decrease was observed in dendritic length.
Conclusion: LFS had a restoring effect on morphological changes in CA1 neurons of kindled animals. This effect may be considered a mechanism for therapeutic action of deep brain stimulation in seizure.
Article Type: Original Research |
Subject:
Anatomical sciences
Received: 2024/05/8 | Accepted: 2024/05/19
Received: 2024/05/8 | Accepted: 2024/05/19
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |