Volume 26, Issue 3 (2023)
mjms 2023, 26(3): 51-56 |
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Ethics code: IR.MODARES.REC.1397.115
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Soodi M, Ghahramani S. Serum Paraoxonase and Arylesterase activity after acute chlorpyrifos poisoning in rat. mjms 2023; 26 (3) :51-56
URL: http://mjms.modares.ac.ir/article-30-77378-en.html
URL: http://mjms.modares.ac.ir/article-30-77378-en.html
1- Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University , soodi@modares.ac.ir
2- Department of Toxicology, Faculty of medical sciences, Tarbiat Modares University
2- Department of Toxicology, Faculty of medical sciences, Tarbiat Modares University
Abstract: (999 Views)
Introduction: Paraoxonase 1 (PON1) is a high-density lipoprotein-associated enzyme with both aryl esterase and lactonase activity, and it possesses significant antioxidant and anti-inflammatory properties. PON1 hydrolyzes the active metabolites of several organophosphorus (OP) insecticides, including parathion, diazinon, and chlorpyrifos. This widely studied enzyme is recognized for its protective role against organophosphate poisoning and vascular diseases, as well as its potential as a biomarker for conditions related to oxidative stress, inflammation, and liver disease. However, limited knowledge exists regarding PON1 activity status following acute organophosphate intoxication. The aim of the present study was to investigate changes in serum PON1 activity after acute chlorpyrifos poisoning and its relationship with acetylcholinesterase (AChE) activity.
Method: Rats were orally given a single dose of chlorpyrifos (160 mg/kg), and blood samples were collected before treatment, as well as at 6 and 96 hours post-treatment. We measured serum cholinesterase, paraoxonase, and arylesterase activity of PON1.
Results: Signs of OP poisoning, including miosis, salivation, tremors, fasciculation, and paralysis, were observed following intoxication, accompanied by a significant inhibition of AChE activity. All symptoms resolved after 48 hours, and AChE activity returned to baseline levels at 96 hours. In contrast, paraoxonase and arylesterase activities progressively increased after 6h and 96h treatment respectively.
Conclusion: The results of our study indicated that serum cholinesterase activity and paraoxonase activity negatively associated in OP poisoning. Based on these findings, monitoring paraoxonase activity after OP intoxication may serve as a valuable biomarker for assessing intoxication.
Method: Rats were orally given a single dose of chlorpyrifos (160 mg/kg), and blood samples were collected before treatment, as well as at 6 and 96 hours post-treatment. We measured serum cholinesterase, paraoxonase, and arylesterase activity of PON1.
Results: Signs of OP poisoning, including miosis, salivation, tremors, fasciculation, and paralysis, were observed following intoxication, accompanied by a significant inhibition of AChE activity. All symptoms resolved after 48 hours, and AChE activity returned to baseline levels at 96 hours. In contrast, paraoxonase and arylesterase activities progressively increased after 6h and 96h treatment respectively.
Conclusion: The results of our study indicated that serum cholinesterase activity and paraoxonase activity negatively associated in OP poisoning. Based on these findings, monitoring paraoxonase activity after OP intoxication may serve as a valuable biomarker for assessing intoxication.
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