Volume 27, Issue 1 (2024)                   mjms 2024, 27(1): 45-52 | Back to browse issues page

Ethics code: IR.ACECR.AEC.1401.005

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Satraian L, Fallah S, Musavi R S. Development of a Heterotopic Zebrafish Model for Real-Time Observation of Retinoblastoma Tumor Growth. mjms 2024; 27 (1) :45-52
URL: http://mjms.modares.ac.ir/article-30-77744-en.html
Development of a Heterotopic Zebrafish Model for Real-Time Observation of Retinoblastoma Tumor Growth
Leila Satraian 1, Shaghayegh Fallah2 , Reyhaneh Sadat Musavi3
1- 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran , Lsatarian@gmail.com
2- 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
3- 1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. 2. Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Abstract:   (909 Views)
Introduction: Retinoblastoma, the most prevalent pediatric eye cancer, arises from mutations in the RB1 gene, leading to the uncontrolled proliferation of retinal cells. This study introduces a heterotopic retinoblastoma model utilizing zebrafish, focusing on injecting the Y79 retinoblastoma cell line into the vitreous cavity for real-time tumor observation. Methods: By leveraging the transparent embryos and rapid eye development of zebrafish, we tracked the establishment and growth of fluorescently labeled tumors. Results: Results confirm tumor formation within three days, underscoring the model's relevance for in vivo studies. The zebrafish model capitalizes on the ease of maintenance, transparency for direct visualization, and genetic tractability, offering significant potential for high-throughput screening and therapeutic assessments. Conclusion: As the field progresses, this model promises to enhance our understanding of retinoblastoma biology and facilitate the discovery of effective treatments, addressing the critical need for innovative approaches in pediatric oncology
Keywords: Retinoblastoma, Pediatric ocular tumor, Y79, Orthotopic Transplantation, Zebrafish model.
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Article Type: Original Research | Subject: Cellular and Molecular Neuroscience
Received: 2024/10/30 | Accepted: 2024/11/3
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