Volume 28, Issue 2 (2025)
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Ethics code: IR.NIGEB.EC.1400.12.10.G
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Jalalifar A, Safavi L, Mohammadi R, Moradifard S, Banoei M, Khalilollahi M et al . Investigating the possible roles of mutations in axin1 and axin2 genes in colorectal cancer. mjms 2025; 28 (2)
URL: http://mjms.modares.ac.ir/article-30-79732-en.html
URL: http://mjms.modares.ac.ir/article-30-79732-en.html
Atiye Jalalifar1 
, Leila Safavi2 , Ramtin Mohammadi3 
, Shirin Moradifard4 , Mahdieh Banoei5 , Mohammad Khalilollahi2 , Shahla Mohammad Ganji 6
, Leila Safavi2 , Ramtin Mohammadi3 , Shirin Moradifard4 , Mahdieh Banoei5 , Mohammad Khalilollahi2 , Shahla Mohammad Ganji 6
1- Department of Molecular Medicine, Medical Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
2- Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3- 1. Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.2. Medical Biotechnology and Bioinformatics Research Group (MBBRG), Universal Scientific Education And Research Network (USERN), Tehran, Iran.
4- Hudson Institute of Medical Research, Monash University, Melbourne, VIC, Australia
5- Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
6- Department of Molecular Medicine, Medical Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. ,shahla@nigeb.ac.ir
2- Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
3- 1. Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.2. Medical Biotechnology and Bioinformatics Research Group (MBBRG), Universal Scientific Education And Research Network (USERN), Tehran, Iran.
4- Hudson Institute of Medical Research, Monash University, Melbourne, VIC, Australia
5- Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
6- Department of Molecular Medicine, Medical Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. ,
Abstract: (217 Views)
Introduction: Colorectal cancer (CRC) is one of the leading cancers, following skin, breast, and stomach cancers. This study aimed to investigate the relationship between mutations in axin1 and axin2 in association with CRC.
Methods: Our study contains 147 fresh frozen samples from CRC patients, 25 normal samples, and 3 cell lines, including HT29, SW480, and CACO-2. The chosen SNPs from databases are placed in exon 5 of axin1, in exon 2 of axin1, and in exon 7 of axin2. By PCR-RFLP method, mutated samples were identified and sequenced.
Results: The results showed that mutations in the single-nucleotide polymorphism (SNP) in axin2 were observed in 1 out of 147 patient samples (0.68%). In the three sequences examined in axin2 (exon 7), mutations in SNP with rs79024445 at A2052C were observed. Statistical analysis of clinical and pathological data of patients showed a significant relationship between the tumor size factor and grade of cancer (P=0.016) as well as the degree of tumor diffusion to the lymph nodes factor with a grade of cancer (P=0.001).
Conclusion: The multi-factorial nature of cancer, the high genetic diversity of the Iranian population, and the limited statistical population could affect these outcomes. The observed mutations in each sample can also indicate the importance of personalized medicine in studying diseases.
Methods: Our study contains 147 fresh frozen samples from CRC patients, 25 normal samples, and 3 cell lines, including HT29, SW480, and CACO-2. The chosen SNPs from databases are placed in exon 5 of axin1, in exon 2 of axin1, and in exon 7 of axin2. By PCR-RFLP method, mutated samples were identified and sequenced.
Results: The results showed that mutations in the single-nucleotide polymorphism (SNP) in axin2 were observed in 1 out of 147 patient samples (0.68%). In the three sequences examined in axin2 (exon 7), mutations in SNP with rs79024445 at A2052C were observed. Statistical analysis of clinical and pathological data of patients showed a significant relationship between the tumor size factor and grade of cancer (P=0.016) as well as the degree of tumor diffusion to the lymph nodes factor with a grade of cancer (P=0.001).
Conclusion: The multi-factorial nature of cancer, the high genetic diversity of the Iranian population, and the limited statistical population could affect these outcomes. The observed mutations in each sample can also indicate the importance of personalized medicine in studying diseases.
Article Type: Original Research |
Subject:
Cancer Research
Received: 2025/02/28 | Accepted: 2025/03/16
Received: 2025/02/28 | Accepted: 2025/03/16
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