Volume 17, Issue 4 (2015)
mjms 2015, 17(4): 53-61 |
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Masti Y, Saadsti M, Nazarian S, Fasihi Ramandi M, Asadi M, Arefpour M. Production of Antibody against CtxB Recombinant Protein based on Nanofibers and Nanocapsules. mjms 2015; 17 (4) :53-61
URL: http://mjms.modares.ac.ir/article-30-8369-en.html
URL: http://mjms.modares.ac.ir/article-30-8369-en.html
Yousef Masti1 
, Mojtaba Saadsti 1, Shahram Nazarian1 , Mahdi Fasihi Ramandi2 , Morteza Asadi1 , Mohammadali Arefpour1
, Mojtaba Saadsti 1, Shahram Nazarian1 , Mahdi Fasihi Ramandi2 , Morteza Asadi1 , Mohammadali Arefpour1
1- Department of Biological Sciences, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran
2- Molecular biology research center, Baqiyatallah university of medical sciences, Tehran, Iran
2- Molecular biology research center, Baqiyatallah university of medical sciences, Tehran, Iran
Abstract: (9672 Views)
Objectives: CtxB (Cholera toxin B subunit) contributes to a vaccine's efficacy by stimulating production of the anti-CtxB antibody. Various attempts have been made to increase production of this antibody. Chitosan is a mucoadhesive polysaccharide that has tremendous potential for oral vaccine delivery in terms of its exclusive features that include biocompatibility, biodegradability, high charge density and non-toxicity. We investigated the potential for chitosan nanofibers and nanocapsules as novel carrier systems for the oral delivery of CtxB.
Methods: Antigen-containing chitosan nanofibers were prepared by electrospinning a chitosan/AcOH solution. Encapsulation of the antigen inside the chitosan nanofibers was confirmed through infrared spectroscopy analysis (FTIR). Guinea pigs were immunized with free antigen and CtxB antigen or antigen alone by direct administration of antigen-containing chitosan nanofibers into the buccal cavity. Serum immunoglobulin G (IgG) and intestinal immunoglobulin A (IgA) antibody responses were determined
Results: The results indicated that antigen in the chitosan nanofibers or nanocapsules elicited very high IgA and IgG responses. No detectable IgA and IgG responses were obtained after oral immunization with CtxB. The results of the antibody titer were analyzed using the ANOVA and LSD tests.
Conclusion: CtxB inside the nanofiber increased antibody production when administered orally. This system might be used for delivery of other antigens.
Article Type: Original Manuscipt |
Subject:
Recombinant Vaccine
Received: 2014/04/9 | Accepted: 2014/10/4
Received: 2014/04/9 | Accepted: 2014/10/4
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