Study on antibiotic resistance profile and multiple antibiotic resistance index (MAR Index) in the Campylobacter spp. isolates from domestic animals and water

Document Type : Original Research

Authors
S. O. Ghasemian 1
H. Mahmoodipour 2
M. Gholami-Ahangaran 3
1 Department of Veterinary Medicine, Behbahan Branch, Islamic Azad University, Behbahan, Iran
2 Department of Nursing and Midwifery, Behbahan Branch, Islamic Azad University, Behbahan, Iran
3 Department of Clinical Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract
The purpose of this study was comparison study on antibiotic resistance profile and multiple antibiotic resistance index (MAR Index) in the Campylobacter spp. isolates from domestic animals and water. To performing the study, 392 fecal and water samples were collected from poultry (182), cow (141), sheep and goat (41) and tap water (28). All samples were subjected for isolation of Campylobacter spp. using pre-treatment-Kapandis Baseri (prêt KB) method and the isolates were confirmed by sequencing of 16srRNA genes. Furthermore, Campylobacter isolates were assessed for antibiotic resistance profile and multiple antibiotic resistance index (MAR Index) by using disk diffusion method. The results indicated that Campylobacter spp. isolated from 50 samples. The isolation rate was highest in poultry (37/50) and lowest in goat (2/50). 36 isolates were identified as Campylobacter jejuni and the rest (14 isolates) were identified as Campylobacter coli. All of C. jejuni and C. coli isolates found resistant to amoxicillin/clavulanic, erythromycin and chloramphenicol and all sensitive to ciprofloxacin, kanamycin, gentamicin, streptomycin, tobramycin, tetracycline and imipenem. 36% of C. jejuni and 14% of C. coli had multiple antibiotic resistance index 0.2 and upper. Therefore, based on foregoing evidence, all of the isolates were resistant to antibiotics, therefore, human infection with Campylobacter spp. via utilization of animal origin products is possible.

Keywords

Subjects

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Pathobiology Reserach
Volume 25, Issue 3
April 2022
Pages 6-14