Introducing a method for implantation of stimulating electrodes in olfactory epithelium for brain stimulation

Document Type : Original Research

Authors
S. R. Eftekhari 1
N. Heidari 2
F. Bakhtyarzadeh 1
1 Tarbiat Modares university
2 Tarbiat modares University
Abstract
Olfactory system may be considered as a target for deep brain stimulation. In experimental researches, it is difficult to insert an electrode in the olfactory epithelium (OE) of laboratory animals, including mice, through nasal cavity. Therefore, it is necessary to find a method for insertion of electrodes in the OE. In the present study we introduce a new method for electrode implantation in mice OE. Male C57BL/6 mice, aged 3-4 months were used. Animals were anesthetized and a pair of bipolar electrodes were inserted into the OE through a hole on the nasal bone. Field potential recordings were made from olfactory bulb and confirmed the electrode positions in OE, so that OE stimulation led to field excitatory post synaptic potentials (fEPSP) in olfactory bulb. In addition, considering the connection between olfactory system and hippocampus, the fEPSPs were also recorded in the hippocampus after a long synaptic delay. On the whole, we introduced a method for OE stimulation in mice as a common laboratory animal. This methos may be suitable in deep brain stimulation experiments in different animal models of neurological diseases.

Keywords

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Pathobiology Reserach
Volume 25, Issue 4
October 2022
Pages 13-22