Volume 22, Issue 4 (2019)
mjms 2019, 22(4): 197-202 |
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Moghadam P, Khadivi Derakhshan F. Isolation and Identification of Halophilic Bacteria Producing Asparaginase Enzyme from Urmia Lake. mjms 2019; 22 (4) :197-202
URL: http://mjms.modares.ac.ir/article-30-38082-en.html
URL: http://mjms.modares.ac.ir/article-30-38082-en.html
1- Department of Microbiology, Faculty of Science, Urmia Branch, Islamic Azad University, Urmia, Iran
2- Department of Biology, Faculty of Science, Urmia Branch, Islamic Azad University, Urmia, Iran ,khadivi.d.f@gmail.com
2- Department of Biology, Faculty of Science, Urmia Branch, Islamic Azad University, Urmia, Iran ,
Abstract: (2859 Views)
Aims: The present study was aimed to investigate isolation and identification of halophil bacteria producing asparaginase from Urmia Lake and the effect of different salt concentrations and temperature on asparaginase production.
Materials & Methods: The water and mud samples from Urmia Lake were cultivated in MH and SWN media for isolation of halotolerant bacteria. Then, the asparaginase production rate was investigated in saline M9. The asparaginase production rate by isolates was investigated in different salt concentrations (10, 20, 30, and 40%) and temperatures (30, 35, 37, 45, and 50°C). Two isolates with a high production rate of asparaginase were stained by gram stain method and were identified by 16S rRNA sequencing.
Findings: In the present study, 57 isolates were obtained which 10 isolates showed asparaginase production. Among these, 2 isolates (A2 and A3) were selected as superior isolates. The isolate A3 has shown the largest halo (54mm) at 35°C and 10% salt concentration. Moreover, the isolate A2 has shown the largest halo (56mm) at 35°C and 40% salt concentration. The 16SrRNA sequencing showed that the A2 isolate was similar 98.9% to the Halomonas elongata strain, and the A3 isolate was similar 100% to the Bacillus aryabhattai strain.
Conclusion: The bacteria isolated from Urmia Lake are potential source of asparaginase enzyme. H. elongate and B. aryabhattai strains isolated from Urmia Lake can be used to production of asparaginase without side effects for cancer patients.
Materials & Methods: The water and mud samples from Urmia Lake were cultivated in MH and SWN media for isolation of halotolerant bacteria. Then, the asparaginase production rate was investigated in saline M9. The asparaginase production rate by isolates was investigated in different salt concentrations (10, 20, 30, and 40%) and temperatures (30, 35, 37, 45, and 50°C). Two isolates with a high production rate of asparaginase were stained by gram stain method and were identified by 16S rRNA sequencing.
Findings: In the present study, 57 isolates were obtained which 10 isolates showed asparaginase production. Among these, 2 isolates (A2 and A3) were selected as superior isolates. The isolate A3 has shown the largest halo (54mm) at 35°C and 10% salt concentration. Moreover, the isolate A2 has shown the largest halo (56mm) at 35°C and 40% salt concentration. The 16SrRNA sequencing showed that the A2 isolate was similar 98.9% to the Halomonas elongata strain, and the A3 isolate was similar 100% to the Bacillus aryabhattai strain.
Conclusion: The bacteria isolated from Urmia Lake are potential source of asparaginase enzyme. H. elongate and B. aryabhattai strains isolated from Urmia Lake can be used to production of asparaginase without side effects for cancer patients.
References
1. 1Kornbrust BA, Stringer MA, Lange NE, Hendriksen HV. Asparaginase-an enzyme for acrylamide reduction in food products. In: Whitehurst R, Oort MV, editors. Enzymes in food technology. 2nd Edition. Hoboken: Blackwell Publishing Ltd; 2010. pp. 59-87. [Link] [DOI:10.1002/9781444309935.ch4]
2. Warangkar SC, Khobragade CN. Screening, enrichment and media optimization for L-asparaginase production. J Cell Tissue Res. 2009;9(3):1963-8. [Link]
3. Hatanaka T, Usuki H, Arima J, Uesugi Y, Yamamoto Y, Kumagai Y, et al. Extracellular production and characterization of two Streptomyces L-asparaginases. Appl Biochem Biotechnol. 2011;163(7):836-44. [Link] [DOI:10.1007/s12010-010-9087-9]
4. Shah AJ, Karadi RV, Parkesh PP. Isolation, optimization and production of L-asparaginase from coliform bacteria. Asian J Biotechnol. 2010;2(3):169-77. [Link] [DOI:10.3923/ajbkr.2010.169.177]
5. Chandrasekaran M, Rajeev Kumar S. Marine microbial enzymes. Biotechnol. In: Doelle HW, Stefan Rokem J, Berovic M, editors. Paris: EOLSS Publications; 1997. pp. 47-79. [Link]
6. Saptarshi SD, Lele SS. Application of evolutionary optimization technique in maximizing the recovery of L-asparaginase from E. caratovovora MTCC 1428. Glob J Biotech Biochem. 2010;5(2):97-105. [Link]
7. Izadpanah Qeshmi F, Javadpour S, Malekzadeh K, Tamadoni Jahromi S, Rahimzadeh M. Persian gulf is a bioresource of potent L-asparaginase producing bacteria: Isolation & molecular differentiating. Int J Environ Res. 2014;8(3):813-8. [Link]
8. Senthil Kumar M, Selvam K. Isolation and purification of high efficiency L-asparaginase by quantitative preparative continuous-elution SDS PAGE electrophoresis. J Microbial Biochem Technol. 2011;3(5):73-83. [Link] [DOI:10.4172/1948-5948.1000055]
9. Mehrshad M, Amoozegar MA, Yakhchali B, Shahzade Fazeli SA. Biodiversity of moderately halophilic and halotolerant bacteria in the western coastal line of Urmia lake. Biological J Microorg. 2012;1(2):49-70. [Persian] [Link]
10. Jookar Kashi F, Owlia P, Amoozegar MA, Yakhchali B. Culturable prokaryotic diversity of Urmia salt lake. Mod Genet J. 2014;9(3):313-28. [Persian] [Link]
11. Das S, Lyla PS, Khan SA. Marine microbial diversity and ecology: Importance and future perspectives. Curr Sci. 2006;90(10):1325-35. [Link]
12. Ramesh S, Mathivanan N. Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J Microbiol Biotechnol. 2009;25(12):2103-11. [Link] [DOI:10.1007/s11274-009-0113-4]
13. Gulati R, Saxena RK, Gupta R. A rapid plate assay for screening l‐asparaginase producing micro‐organisms. Lett Appl Microbiol. 1997;24(1):23-6. [Link] [DOI:10.1046/j.1472-765X.1997.00331.x]
14. Mahdavi S, Sadeghi Zali M, Farajnia S, Mehmannavaz Y, Isazadeh A. The comparison of bovine fecal and buffy coat samples for diagnosis of Johne's Disease based on PCR. Gene Cell Tissue. 2018;5(2):e79745. [Link] [DOI:10.5812/gct.79745]
15. Vreeland RH, Martin EL. Growth characteristics, effects of temperature, and ion specificity of the halotolerant bacterium Halomonas elongata. Can J Microbiol. 1980;26(7):746-52. [Link] [DOI:10.1139/m80-130]
16. Mahdavi S, Isazadeh A. Lactobacillus casei suppresses hfq gene expression in Escherichia coli O157: H7. Br J Biomed Sci. 2019;76(2):92-4. [Link] [DOI:10.1080/09674845.2019.1567903]
17. Ebrahiminezhad A, Rasoul-Amini S, Ghasemi Y. l-Asparaginase production by moderate halophilic bacteria isolated from Maharloo Salt Lake. Indian J Microbiol. 2011;51(3):307-11. [Link] [DOI:10.1007/s12088-011-0158-6]
18. Yazdani Y, Mobini-Dehkordi M, Rastegari A. Isolation and identification of native Iranian L-asparaginase producing bacteria. J Microb World. 2012;5(1-2):39-46. [Persian] [Link]
19. Zolfaghar M, Amoozegar MA, Khajeh K. Production of extracellular L-asparaginase by halophilic bacterium vibrio sp. Biol J Microorg. 2016;5(18):41-54. [Persian] [Link]
20. Ghorbanmovahed M, Ebrahimipour G, Akhtari J, Marzban A. Production of anti-leukemia L-Asparaginase by a strain of Staphylococcus Isolated from Agricultural Soil. J Mazandaran Univ Med Sci. 2016;25(132):1-2. [Persian] [Link]
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