Volume 22, Issue 4 (2019)                   mjms 2019, 22(4): 181-187 | Back to browse issues page

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Seyed Ahmadian S, Jalili Sh. S, Shirzad H, Sadeghizadeh M. Comparative Evaluation of the Efficacy of Milk and Milk Containing Nano-Curcumin on Lead Toxicity in Huh7-1x-ARE-luc cell-line. mjms. 2019; 22 (4) :181-187
URL: http://mjms.modares.ac.ir/article-30-30672-en.html
1- Nutrition Sciences & Food Technology Research Center, Medical Sciences Branch, Islamic Azad University, Tehran, Iran
2- Research Institute of Police Science & Social Studies, Tehran, Iran
3- Research Institute of Police Science & Social Studies, Tehran, Iran , hadi_shirzad@yahoo.com
4- Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Abstract:   (2725 Views)
Aims: Oxidative substances are chemically reactive molecules and a byproduct of oxidative metabolism. Oxidative stress is one of the most lethal mechanisms in the toxicity of heavy metals such as lead. Since curcumin is an active ingredient in turmeric and has many properties, including antioxidant properties, the present study was conducted to evaluate the effect of milk and milk containing nano-curcumin on lead toxicity and to determine the effective concentration of nano-curcumin in controlling lead toxicity.
Materials & Methods: In the present study, the Huh7-1x-ARE-luc cell line, a biosensor of oxidative stress, was treated with 30μM of lead as a strong oxidant. Then the antioxidant effect of low-fat and high-fat milk (20, 40, and 80μL), nano-curcumin in antioxidant concentrations (4 and 8μM) and simultaneous treatment with the combination of these two antioxidants was tested using Luciferase assay.
Results: Based on statistical analyses, the combination of milk and nano-curcumin (combination of 30μM lead, 20μL milk and 4μM nano-curcumin) was able to significantly reduce lead toxicity at low concentrations of milk compared to the milk without nano-curcumin (combination of 30μM lead and 80μL milk), with RLU of 1266 and 34000, respectively.
Discussion & Conclusion: Nano-curcumin reveals a stronger antioxidant effect compared to milk, and ultimately, the combination of nano-curcumin and milk greatly neutralizes lead toxicity.
Full-Text [PDF 934 kb]   (389 Downloads)    
Article Type: Original Research | Subject: Pharmacology
Received: 2019/03/2 | Accepted: 2019/11/20

1. Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: Current status and future prospects. Japi. 2004;52(794804):794-804. [Link]
2. Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol. 2014;7(2):60-72. [Link]
3. Papanikolaou NC, Hatzidaki EG, Belivanis S, Tzanakakis GN, Tsatsakis AM. Lead toxicity update. A brief review. Med Sci Monit. 2005;11(10):RA329-36. [Link]
4. Lushchak VI. Free radicals, reactive oxygen species, oxidative stress and its classification. Chem Biol Interact. 2014;224:164-75. [Link]
5. Malekirad AA, Fani A, Abdollahi M, Oryan SH, Babapour V, Shariatzade SM, et al. Blood-urine and cognitive-mental parameters in mine workers exposed to lead and zinc. Arak Med Univ J. 2011;13(4):106-13. [Persian] [Link]
6. Noriega GO, Tomaro ML, Del Batlle AM. Bilirubin is highly effective in preventing in vivo δ-aminolevulinic acid-induced oxidative cell damage. Biochimica et Biophysica Acta. 2003;1638(2):173-8. [Link]
7. Flora SJ, Flora G, Saxena G, Mishra M. Arsenic and lead induced free radical generation and their reversibility following chelation. Cell Mol Biol. 2007;53(1):26-47. [Link]
8. Patil A, Bhagwat V, Patil J, Dongre N, Ambekar J, Jailkhani R, et al. Effect of lead (Pb) exposure on the activity of superoxide dismutase and catalase in battery manufacturing workers (BMW) of Western Maharashtra (India) with reference to heme biosynthesis. Int J Environ Res Public Health. 2006;3(4):329-37. [Link]
9. Perez RR, Sousa CA, Vankeersbilck T, Machado MD, Soares EV. Evaluation of the role of glutathione in the lead-induced toxicity in Saccharomyces cerevisiae. Curr Microbiol. 2013;67(3):300-5. [Link]
10. Tapia E, Soto V, Ortiz-Vega KM, Zarco-Márquez G, Molina-Jijón E, Cristóbal-García M, et al. Curcumin induces Nrf2 nuclear translocation and prevents glomerular hypertension, hyperfiltration, oxidant stress, and the decrease in antioxidant enzymes in 5/6 nephrectomized rats. Oxida Med Cell Longev. 2012;2012:269039. [Link]
11. Trujillo J, Chirino YI, Molina-Jijón E, Andérica-Romero AC, Tapia E, Pedraza-Chaverrí J. Renoprotective effect of the antioxidant curcumin: Recent findings. Redox Biol. 2013;1(1):448-56. [Link]
12. Kavoosi S, Shirzad H, Jalili Sh, Sadeghizadeh M. Monitoring the nanocurcumin effect on lead exposure in the Huh7-1x-ARE-luc cell line. Pathobiol Res. 2016;19(3):73-88. [Persian] [Link]
13. Motahari P, Sadeghizadeh M, Behmanesh M, Sabri S, Zolghadr F. Generation of stable ARE-driven reporter system for monitoring oxidative stress. DARU J Pharm Sci. 2015;23(1):38. [Link]
14. Tahmasebi Mirgani M, Isacchi B, Sadeghizadeh M, Marra F, Bilia AR, Mowla SJ, et al. Dendrosomal curcumin nanoformulation downregulates pluripotency genes via miR-145 activation in U87MG glioblastoma cells. Int J Nanomed. 2014;9(1):403-17. [Link]
15. Babaei E, Sadeghizadeh M, Hassan ZM, Hosseinpour Feizi MA, Najafi F, Hashemi SM. Dendrosomal curcumin significantly suppresses cancer cell proliferation in vitro and in vivo. Int Immunopharmacol. 2012;12(1):226-34. [Link]
16. Tahmasebi Birgani M, Erfani-Moghadam V, Babaei E, Najafi F, Zamani M, Shariati M, et al. Dendrosomal nano-curcumin; The novel formulation to improve the anticancer properties of curcumin. Prog Biol Sci. 2015;5(2):143-58. [Link]
17. Kavoosi S, Shirzad H, Jalili Sh, Sadeghizadeh M, Motahari P. Monitoring lead toxicity by Huh7-1x-ARE-luc cell line lucifersae biosensor. Modares J Biotechnol. 2019;10(1):125-31. [Persian] [Link]
18. Sies H. Oxidative stress: A concept in redox biology and medicine. Redox Biol. 2015;4:180-3. [Link]
19. Buendia I, Michalska P, Navarro E, Gameiro I, Egea J, Leon R. Nrf2-ARE pathway: An emerging target against oxidative stress and neuroinflammation in neurodegenerative diseases. Pharmacol Ther. 2016;157:84-104. [Link]
20. Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem. 2015;97:55-74. [Link]
21. Fuentes F, Gomez Y, Paredes-Gonzalez X, Barve A, Nair S, Yu S, et al. Nrf2-mediated antioxidant and detoxifying enzyme induction by a combination of curcumin and sulforaphane. J Chin Pharm Sci. 2016;25(8):559-69. [Link]
22. Banerjee A, Kunwar A, Mishra B, Priyadarsini KI. Concentration dependent antioxidant/pro-oxidant activity of curcumin: Studies from AAPH induced hemolysis of RBCs. Chem Biol Interact. 2008;174(2):134-9. [Link]

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